Platelet Shape Change and Mobilization of Cytoplasmic Ionized Calcium Are Mediated by Distinct Binding Sites on Platelets: 5’-p-Fluorosulfonylbenzoyladenosine Is a Weak Platelet Agonist
نویسنده
چکیده
Platelet stimulation with ADP results in several responses. including shape change. increase in cytoplasmic ionized calcium concentration [Ca2 ],, and inhibition of adenylate cyclase. 5’-p-Fluorosulphonyb benzoybadenosine (FSBA), which covalently labels an ADP binding site on platelets. blocks platelet shape change but not the inhibition of cyclic AMP levels by ADP. whereas p-chloromercuribenzenesulfonate (pCMBS). a nonpenetrating thiob reagent, has the opposite effects. We examined the effect of FSBA and pCMBS on ADP-induced increase in [Ca2 ], using platelets loaded with fluorescent Ca2 indicators quin2 and fura-2.
منابع مشابه
ADP-induced platelet shape change and mobilization of cytoplasmic ionized calcium are mediated by distinct binding sites on platelets: 5'-p-fluorosulfonylbenzoyladenosine is a weak platelet agonist.
Platelet stimulation with ADP results in several responses, including shape change, increase in cytoplasmic ionized calcium concentration [Ca2+]i, an inhibition of adenylate cyclase. 5'-p-Fluorosulphonyl benzoyladenosine (FSBA), which covalently labels an ADP binding site on platelets, blocks platelet shape change but not the inhibition of cyclic AMP levels by ADP, whereas p-chloromercuribenzen...
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Platelet stimulation with ADP results in several responses. including shape change. increase in cytoplasmic ionized calcium concentration [Ca2 ],, and inhibition of adenylate cyclase. 5’-p-Fluorosulphonyb benzoybadenosine (FSBA), which covalently labels an ADP binding site on platelets. blocks platelet shape change but not the inhibition of cyclic AMP levels by ADP. whereas p-chloromercuribenze...
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